Post-translational Modification of LipL32 during Leptospira interrogans Infection

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Post-translational Modification of LipL32 during Leptospira interrogans Infection

BACKGROUND Leptospirosis, a re-emerging disease of global importance caused by pathogenic Leptospira spp., is considered the world's most widespread zoonotic disease. Rats serve as asymptomatic carriers of pathogenic Leptospira and are critical for disease spread. In such reservoir hosts, leptospires colonize the kidney, are shed in the urine, persist in fresh water and gain access to a new mam...

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Comparison of Leptospira interrogans infection in the goats and sheep

Background: Most leptospiral infections in sheep and goat are asymptomatic but may result in high fever, abortion, stillbirth, agalactiae. There is a different foraging behavior between sheep and goat that may cause the different prevalence of Leptospira interrogans infection in sheep and goats. OBJECTIVES: The purpose of the present study was to compare the prevalence of L. interrogans antibod...

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Mouse model for sublethal Leptospira interrogans infection.

Although Leptospira can infect a wide range of mammalian species, most studies have been conducted in golden Syrian hamsters, a species particularly sensitive to acute disease. Chronic disease has been well characterized in the rat, one of the natural reservoir hosts. Studies in another asymptomatic reservoir host, the mouse, have occasionally been done and have limited infection to mice younge...

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Expression and characterization of recombinant leptospiral outer membrane protein LipL32 from Leptospira interrogans serovar autumnalis.

Leptospira interrogans serovar autumnalis, a causative agent of leptospirosis in Thailand, was isolated from a patient for DNA extraction and amplification of LipL32 gene by polymerase chain reaction (PCR). The 782 bp PCR product was obtained, which was inserted into pAE plasmid with polyhistidine (His6 tag) to construct pAE-LipL32. This recombinant plasmid was transfected into E. coli BL21 (DE...

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Expression and purification of LipL32 recombinant protein of Leptospira interrogans in different E.coli strains

Background Leptospirosis is a zoonosis caused by the infection of pathogenic bacteria of the genera Leptospira, often transmitted by direct or indirect contact with urine of infected animal hosts [1]. In national territory, leptospirosis is considered an endemic disease, with high incidence in raining seasons, but occurring during the whole year [2]. From all the cases, 15% evolve to the icteri...

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ژورنال

عنوان ژورنال: PLoS Neglected Tropical Diseases

سال: 2014

ISSN: 1935-2735

DOI: 10.1371/journal.pntd.0003280